Lowland League leaders East Kilbride stunned second-tier Ayr United to reach the semi-finals of the SPFL Trust Trophy.
Championship sides Dunfermline Athletic and Livingston progressed to the last four, but league rivals Ayr lost 3-2 at K Park Stadium.
Striker John Robertson put fifth-tier East Kilbride ahead after only a minute and midfielder Joao Balde headed a second 11 minutes later.
Shots from midfielders Jay Henderson and Marco Rus drew Ayr level with two goals midway through the second half.
However, striker Cameron Elliott put the hosts back ahead within three minutes as East Kilbride moved into the Scottish Challenge Cup semi-finals for the first time.
Meanwhile, Livingston edged out second-tier rivals Greenock Morton 2-1.
Midfielder Reece McAlear shot the hosts ahead just before the break, striker Stevie May fired the second three minutes from the end of normal time and forward Austin Samuels’ stoppage-time drive in reply was not enough to deny Livingston.
Dunfermline made hard work of beating League 1 visitors Alloa Athletic, with midfielder Lewis McCann’s 37th-minute goal all that separated the sides.
Championship side Queen’s Park host Rangers B in the final quarter-final, which will be played on 3 December, to decide the full line-up for the semis at the end of January.