England’s Morgan Gibbs-White, Ezri Konsa and Kobbie Mainoo have been ruled out of England’s Nations League games in October.
The Three Lions host Greece at Wembley on Thursday before playing Finland in Helsinki on Sunday.
Interim manager Lee Carsley is not planning to add anybody to the squad in their place.
The other 22 players named in his initial squad have all reported to St George’s Park.
Nottingham Forest midfielder Gibbs-White, who made his England debut last month, was injured while having a shot blocked in their 1-1 draw with Chelsea.
Aston Villa defender Konsa suffered a hamstring injury in Sunday’s goalless draw with Manchester United.
United midfielder Mainoo was replaced in the closing stages of that match.